Ensuring steroid-free food supplements

Ensuring steroid-free food supplements – Shimadzu News 02 / 2020

Identification of steroids in pharmaceuticals and food supplements with LCMS-8045

Authors: Dr. Gerasimos Liapatas & Dr. Manos Barbounis (Applications Department of N. Asteriadis S.A.)

Anabolic steroids are often encountered in samples of nutritional supplements and pharmaceutical preparations, either due to doping-related activities [1] or cross-contamination issues during production [2].
Developing methods to identify steroids in such samples can be challenging, since the method should be able to monitor numerous steroids with large differences in terms of polarity.

Compounds to be monitored comprise relatively polar steroids such as anastrozole as well as low polarity steroid esters which have been occurring with increasing frequency in food supplement samples.
The method developed includes testing for testosterone caproate and testosterone isocaproate, a challenging set of isomers which were partially separated (Resolution 0.6 as per Eur.Ph.) enabling the analytical method to identify which of the two isomers is contained in the sample. With respect to tibolone, three MRM transitions are reported with no need to resort to derivatization, which is usually the case with tibolone and its metabolites. Finally, a comprehensive rationale is provided for choosing MRM transitions for steroid esters, based on fragments of the free steroid read the full article on Shimadzu’s News 02/2020 p.11-13 …

Antec Scientific – Azithromycin a treatment for COVID-19?

Azithromycin a treatment for COVID-19?

Antec Scientific contribution to the treatment of COVID-19 with Azithromycin

Leiden, NL (8. April 2020). You may be wondering what Antec Scientific ALEXYS™ Antibiotics Analyzer has to do with the coronavirus SARS-CoV-2 first occurred in Wuhan, China, causing a worldwide pandemic respiratory disease COVID-19.
As a matter of fact secondary bacterial infections (co-infections, superinfections) are a major risk factor for adverse COVID-19 outcomes.  A recent study from China published in the Lancet (Zhou et al., 2020) found that 27 out of 28 patients with bacterial co-infections died (96%). Staphylococcus aureus is the main cause of secondary infections in influenza but Streptococcus pneumoniae and Haemophilus influenzae are also commonly found, although it is not yet known which pathogens are associated with secondary infections in COVID-19, except for the known Acinetobacter Baumannii, a typically hospital acquired (nosocomial) infection.
Almost all hospitalized patients receive immediately empirical antibiotic treatment to strengthen the immune system and to reduce further complications by ventilation associated bacterial co-infections and to reduce mortality. Azithromycin is one of the antibiotics that has been widely applied for light as well as heavily infected COVID-19 patients (China, Italy, Spain and now worldwide). In a new French study published in Intl. Journal Antimicrob Agents (Gautret et al., 2020), early evidence was also found that the combination of hydroxychloroquine, a popular anti-malarial drug and antibiotic azithromycin could be especially effective in treating the COVID-19 coronavirus and reducing the duration of the virus in patients.