UV-1280

UV-VIS Spectrophotometer

Designed by the leaders in UV-Visible Spectroscopy for molecular absorption quantitative analysis, the UV-1280 Multipurpose UV-Visible Spectrophotometer offers wavelength scanning from 190-1100 nm.

This lower-cost, high-quality instrument is ideal for applications ranging from routine environmental and food quality testing to life science analyses.

The easy-to-see LCD and buttons enable user-friendly, intuitive measurement, instrument validation, and printing operations.

Equipped with a range of programs, the UV-1280 can be used for everything from photometric, spectral, and kinetics measurements to DNA/protein and high-level multi-component quantitation.

Photometric Mode Measures the absorbance or transmittance at a single wavelength or multiple (up to eight) wavelengths. The instrument is also capable of simple quantitation using the K-factor method. For a multiple-wavelength measurement, calculations can be performed on the data obtained for up to four wavelengths, including the calculation of the difference between, or ratio of, the measurements obtained for two wavelengths.
Spectrum Mode A sample spectrum is recorded using wavelength scanning. Repeat scans let you follow sample changes over time. Zoom in on the finished spectrum for a better view; subsequently, use the peak/valley pick function to select maxima and minima and perform a wide variety of data processing functions.
Quantitation Mode Generates a calibration curve from the measurement of standards, and then calculates the concentrations of unknowns. Allows various combinations of wavelength number (1 to 3 wavelengths and derivatives) and calibration curves (K-factor and first-to-third order).
Kinetics Mode Measures absorbance changes as a function of time, and obtains the enzymatic activity values. The kinetics measurement method automatically calculates the amount of change per minute, and then calculates an activity value from a specified coefficient. The rate measurement method, which determines whether the absorbance is changing linearly, can also be selected. In addition, add the CPS-100 thermoelectrically temperature-controlled cell positioner for measurement of multiple samples in succession.
Time Scan Mode Measures the change in absorbance, transmittance, or energy as a function of time. Add the CPS-100 thermoelectrically temperature-controlled cell positioner for simultaneous measurement of multiple samples under constant-temperature conditions.
Multi-Component
Quantitation Mode
Quantitates up to eight components mixed in a single sample. The calibration equation is determined using pure or mixed components with known values.
Biomethod Mode Determine DNA and protein concentrations with the following quantitation methods using the bioscience/life science program included as standard.

DNA/Protein Quantitation

Quantitates DNA or protein using the absorbance at 260/230 nm or 260/280 nm.

Protein Quantitation

Lowry method

BCA method (method using bicinchoninic acid)

Biuret method

CBB method (method using Coomassie Brilliant Blue G-250)

UV absorption method (direct measurement at 280 nm)

 

For further information about its features, visit Shimadzu official website.

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