PPSQ-51A/53A

The PPSQ-51A/53A Protein Sequencer is a state-of-the-art instrument designed for determining amino acid sequences of proteins and peptides with high precision and efficiency. Combining Edman degradation technology with high-performance liquid chromatography (HPLC), the PPSQ series offers reliable, high-sensitivity protein sequencing for research and pharmaceutical applications.

The PPSQ-51A model is equipped with one reactor, while the PPSQ-53A features three reactors, allowing for continuous sequence analysis of multiple samples. This flexibility makes the system ideal for laboratories requiring high-throughput and reproducible sequencing workflows.

Key Benefits of Edman Degradation for Amino Acid Sequencing

1. Guaranteed N-terminal sequence determination – Ensures precise sequencing from the N-terminal of proteins.
2. No complex sample pre-treatment required – Simplifies the sequencing workflow.
3. Clear differentiation of isobaric amino acids (leucine and isoleucine) – Provides unambiguous identification of structurally similar amino acids.
4. Identification of unknown proteins – Enables sequencing even for proteins not registered in databases, making it ideal for novel protein research.

Advanced Features for Reliable Protein Sequencing

• FDA 21 CFR Part 11 Compliance
  • The LabSolutions PPSQ software ensures full compliance with FDA 21 CFR Part 11, meeting data security, user management, and audit trail
  • Regulatory compliance makes the system suitable for pharmaceutical and biotechnological applications where data integrity is essential.
• Isocratic System for Stable and High-Sensitivity Analysis
  • Baseline Stability: The PPSQ series utilizes isocratic elution to separate PTH-amino acids, significantly improving baseline stability and ensuring consistent, high-sensitivity detection.
  • Retention Time Reproducibility: Isocratic analysis ensures stable retention times, making it easier to identify sequences through substation chromatogram processing.
• Gradient System for High-Sensitivity Analysis
  • A high-sensitivity flow cell enhances detection, making it possible to perform sequential analysis using trace sample amounts.
• Seamless Integration with HPLC and Automated Data Processing
  • After Edman degradation, the stable PTH-amino acids are injected directly into the HPLC system for real-time analysis.
  • The chromatographic data is saved on a PC and processed using specialized sequencing software, allowing for precise amino acid identification and sequence estimation.

Ideal Applications for Protein Research

The PPSQ-51A/53A is an indispensable tool for biological and pharmaceutical research, particularly in:

✔ Protein characterization – Identifying unknown proteins or verifying protein sequences.
✔ Biopharmaceutical development – Ensuring protein purity and structure validation.
✔ Antibody and enzyme research – Analyzing the sequence of therapeutic proteins.
✔ Proteomics and biomarker discovery – Supporting biological and clinical research.

Conclusion

The Shimadzu PPSQ-51A/53A Protein Sequencer is a powerful, high-precision sequencing system designed for efficient, accurate, and regulatory-compliant protein analysis. Its combination of automated Edman degradation, HPLC integration, and advanced data processing makes it an essential tool for laboratories engaged in protein research, drug development, and structural biology.

For detailed specifications and further information, please visit the official Shimadzu website.