Eclipse™

The Eclipse™ Field-Flow Fractionation (FFF) system by Wyatt Technology represents a significant advancement in the separation and characterization of nanoparticles and macromolecules. Designed to handle a diverse array of analytes, including viral particles, liposomes, lipid nanoparticles, emulsions, proteins and their aggregates, polysaccharides, technical polymers, engineered nanoparticles, colloidal suspensions, and chemical mechanical polishing slurries, the Eclipse system offers unparalleled versatility in size-based separations without the need for a stationary phase and with minimal shear forces. ​

Key Features of the Eclipse™ System:

• Versatile Separation Capabilities: The Eclipse system is adept at fractionating a wide range of particles and macromolecules, accommodating sizes from 1 nm to 1000 nm. This broad applicability makes it suitable for various industries and research fields, including biopharmaceuticals, nanotechnology, and materials science. ​
• Integration with Multi-Angle Light Scattering (MALS): Serving as the foundation for FFF-MALS, the Eclipse system enables comprehensive characterization of macromolecules and nanoparticles. This integration facilitates the determination of absolute molar mass, size distributions, and other critical physical properties, providing a holistic understanding of the analytes under investigation. ​
• Dilution Control Module (DCM): An advanced feature of the Eclipse system, the DCM minimizes band broadening and enhances resolution during separation. By controlling dilution, it ensures that sample integrity is maintained, leading to more accurate and reliable results. ​
• Seamless Switching Between FFF and SEC: The Eclipse system offers the flexibility to switch effortlessly between Field-Flow Fractionation and Size-Exclusion Chromatography (SEC). This adaptability allows users to select the most appropriate separation technique based on their specific analytical requirements, thereby optimizing workflow efficiency. ​
• Mobility Add-On for Charge and Zeta Potential Measurements: Expanding its analytical capabilities, the Eclipse system can be equipped with a Mobility add-on to measure electrophoretic mobility, charge, and zeta potential. These measurements are essential for understanding the stability and behavior of colloidal systems and nanoparticles. ​

Applications of the Eclipse™ System:

The Eclipse system’s robust design and advanced features make it particularly suitable for:​

• Biotherapeutic Analysis: Facilitates the separation and characterization of proteins, aggregates, and assemblies, aiding in the development and quality control of biopharmaceutical products. ​
• Nanoparticle Research: Enables detailed studies of engineered nanoparticles, liposomes, lipid nanoparticles, and viral vectors, providing insights into their size distributions and structural properties.
• Polymer Characterization: Supports the analysis of polysaccharides and technical polymers, assisting in the determination of molar mass and conformation, which are critical for material performance evaluations.

By integrating cutting-edge technology with user-friendly features, the Eclipse™ Field-Flow Fractionation system stands as a premier solution for researchers and industry professionals seeking precise and comprehensive separation and characterization of nanoparticles and macromolecules.

For further information about its specifications and features, please visit the official WYATT website.