Photochemical Reactor for the Derivatisation of Aflatoxins with UV-Light
Due to the low aflatoxins limits in food and low inherent fluorescence of aflatoxins B1 and G1, aflatoxin analysis needs to be optimised through derivatisation.
This is done photochemically with the UVE under UV light radiation at 254 nm.
Aflatoxins B1 and G1 are thereby hydroxilated and can then be measured through fluorescence spectrometry. The sensitivity of the measurement increases considerably.
Key advantage of using UVE over electrochemical bromination: the water, present in the eluent, is used as the reagent, hence neither iodine nor HNO3 / KBr are required. In addition, the detector will not be contaminated and no variation in derivatisation will occur. This method is accepted by the AOAC, has been used successfully in collaborative trials and is in use worldwide in accredited laboratories.